02052nas a2200385   4500000000100000000000100001008004100002653000900043653001100052653001400063653001700077100001500094700001300109700001200122700001700134700001300151700001700164700001700181700001300198700001200211700001500223700001400238700001900252700001400271700001700285700001800302700001800320700001700338245011700355856007200472300001100544490000700555520109000562022001401652       2022                            d10asage10agenome10aditerpene10agene cluster1 aChen-Yi Li1 aLei Yang1 aYan Liu1 aZhou-Geng Xu1 aJian Gao1 aYan-Bo Huang1 aJing-Jing Xu1 aHang Fan1 aYu Kong1 aYu-Kun Wei1 aWen-Li Hu1 aLing-Jian Wang1 aQing Zhao1 aYong-Hong Hu1 aYi-Jing Zhang1 aCathie Martin1 aXiao-Ya Chen00aThe sage genome provides insight into the evolutionary dynamics of diterpene biosynthesis gene cluster in plants  uhttps://www.sciencedirect.com/science/article/pii/S2211124722010531  a1112360 v403 a<p>Summary The widely cultivated medicinal and ornamental plant sage (Salvia officinalis L.) is an evergreen shrub of the Lamiaceae family, native to the Mediterranean. We assembled a high-quality sage genome of 480 Mb on seven chromosomes, and identified a biosynthetic gene cluster (BGC) encoding two pairs of diterpene synthases (diTPSs) that, together with the cytochromes P450 (CYPs) genes located inside and outside the&nbsp;cluster, form two expression cascades responsible for the shoot and root diterpenoids, respectively, thus extending BGC functionality from co-regulation to orchestrating metabolite production in different organs. Phylogenomic analysis indicates that the Salvia clades diverged in the early Miocene. In East Asia, most Salvia species are herbaceous and accumulate diterpenoids in storage roots. Notably, in Chinese sage S.&nbsp;miltiorrhiza, the diterpene BGC has contracted and the shoot cascade has been lost. Our data provide genomic insights of micro-evolution of growth type-associated patterning of specialized metabolite production in plants.</p>
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