01841nas a2200241   4500000000100000008004100001653003200042653001900074653001700093653002400110653001600134100002400150700001900174700002100193700001700214700002500231245009800256856007200354300001000426490000700436520114200443022001401585       2017                            d10aChromosome characterization10aKaryo-idiogram10aISSR markers10aGenetic variability10aDNA content1 aClaudio Palma-Rojas1 aCarla Gonzalez1 aBasilio Carrasco1 aHerman Silva1 aHerman Silva-Robledo00aGenetic, cytological and molecular characterization of chia (Salvia hispanica L.) provenances  uhttps://www.sciencedirect.com/science/article/pii/S0305197817300790  a16-210 v733 a<p>Chia (Salvia hispanica L.) is a native plant to southern Mexico and northern Guatemala although in these days also is cultivated in others South American countries such as Bolivia and Argentina. This study describes a genetic, cytological and molecular characterization of nine provenances of Salvia hispanica. The genome size was estimated to be C&nbsp;=&nbsp;0.84&nbsp;pg of DNA. Also a quantitative description of the karyotype is presented. The karyo-idiogram analysis shows that pairs 5 and 6 of the chromosomes are in the limit between submetacentric and subtelocentric types. When the nine provenances were studied using ISSR markers we were able to observe a very low genetic variability for the ISSR loci analysed (h&nbsp;=&nbsp;0.08–0.15; I&nbsp;=&nbsp;0.10–0.23; P&nbsp;=&nbsp;9.3–23.5%). Selfing and reduced genetic background produced by genetic drift and human selection, might explain in part the scarce genetic differentiation found among provenances. These genetic characterizations are part of the basic genetic information, which should be considered for improvement and germplasm conservation programs.</p>
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